Unbound MEDLINE

Lymphocyte production of gamma-interferon as a test for non-tuberculous mycobacterial lymphadenitis in childhood.

Abstract

Infection with Mycobacterium avium, M. intracellulare and M. scrofulaceum (MAIS) organisms in normal children may result in cervical lymphadenopathy. There is a poor response to anti-tuberculous therapy and surgical excision of infected nodes is necessary. The diagnosis therefore requires consideration in children with cervical lymphadenopathy. A simple in vitro assay is described which may be useful for diagnosis prior to excision. Whole blood is cultured with M. avium purified protein derivative. After 24 h the plasma is removed and the concentration of gamma interferon (IFN-gamma) quantified by a radioimmunometric assay. In a prospective study of 38 children with neck lesions, 16 showed MAIS organisms isolated from cultures of excised tissue. The level of IFN-gamma produced by peripheral blood lymphocytes from children in the MAIS group was significantly greater when compared with children having operations for neck lesions that were not due to MAIS organisms (P < 0.001).

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  • Authors

    Davidson PM, Creati L, Wood PR, Roberton DM, Hosking CS

    Institution

    Department of Surgery, Royal Children's Hospital, Melbourne, Australia.

    Source

    European journal of pediatrics 152:1 1993 Jan pg 31-5

    MeSH

    Adolescent
    Antibodies, Bacterial
    Child
    Child, Preschool
    Female
    Humans
    Infant
    Interferon-gamma
    Lymphatic Diseases
    Lymphocytes
    Male
    Mycobacterium Infections, Atypical
    Mycobacterium avium
    Mycobacterium avium-intracellulare Infection
    Mycobacterium scrofulaceum
    Neck
    Prospective Studies
    Tuberculin

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    8444203