Unbound MEDLINE

Rapid diagnosis of porcine epidemic diarrhea virus infection by polymerase chain reaction.

Abstract

The diagnosis of porcine epidemic diarrhea virus (PEDV) infection in the laboratory is rather fastidious because of difficulties in virus propagation. The feasibility of virus propagation in vivo is also limited by the handling of a number of samples at the same time. In this study, the detection of PEDV by reverse transcription polymerase chain reaction (RT-PCR) is described. The RT-PCR could detect up to 10(4) TCID50/ml of PEDV and did not show any cross reaction with transmissible gastroenteritis virus or porcine rotavirus. Using this method, the detection of PEDV in experimentally inoculated piglets was possible as early as one day after inoculation. These results suggest that the RT-PCR could be applicable for a rapid diagnosis of PEDV infection.

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  • FREE Publisher Full Text
  • Authors

    Kweon CH, Lee JG, Han MG, Kang YB

    Source

    The Journal of veterinary medical science / the Japanese Society of Veterinary Science 59:3 1997 Mar pg 231-2

    MeSH

    Animals
    Base Sequence
    Coronavirus
    Coronavirus Infections
    Cross Reactions
    DNA, Viral
    Diagnosis, Differential
    Feces
    Polymerase Chain Reaction
    Rotavirus
    Swine
    Swine Diseases
    Transmissible gastroenteritis virus

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    9101487