| Title | Differential inhibition of Maillard protein fluorescence by nitric oxide donors. | | Author(s) | Suárez G, Wang XH | | Institution | Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595, USA. | | Source | Nitric Oxide 1998; 2(6):475-80. | | MeSH | Fluorescence
Glucose
Maillard Reaction
Nitric Oxide Donors
Penicillamine
Reactive Oxygen Species
S-Nitroso-N-Acetylpenicillamine
Serum Albumin, Bovine
| | Abstract | The nitric oxide donors nitroprusside (NP) and S-nitroso-N-acetylpenicillamine (SNAP) were added repeatedly over a prolonged period into a protein fructation system of 0.05 M fructose and BSA. These additions inhibited Maillard reaction advanced-stage fluorescence generation in a dose-dependent manner without affecting initiation of glycation. NP caused 66% inhibition whereas SNAP caused only 30% inhibition at maximum dose. The lower inhibition by SNAP possibly reflects an interference caused by N-acetylpenicillamine and mediated by a metal-dependent enhanced free-radical generation. We propose that the inhibition of fluorescence results from mutual annihilation between nitric oxide and free radicals, such as OH*, produced during fructation. In vivo generated nitric oxide may play a protective role in cells against the deleterious effect of free radicals that are associated with the augmented fructose autoxidation and fructation that occurs in diabetes. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 10342491 |
|
