| Title | Hypercoagulable state mutation analysis in white patients with early first-trimester recurrent pregnancy loss. | | Author(s) | Kutteh WH, Park VM, Deitcher SR | | Institution | Department of Obstetrics and Gynecology, The University of Tennessee, Memphis 38163-2116, USA. | | Source | Fertil Steril 1999 Jun; 71(6):1048-53. | | MeSH | Abortion, Habitual
Antibodies, Anticardiolipin
Autoantibodies
Blood Coagulation Disorders
Case-Control Studies
DNA Mutational Analysis
Factor V
Female
Humans
Methylenetetrahydrofolate Reductase (NADPH2)
Mutation
Oxidoreductases Acting on CH-NH Group Donors
Phosphatidylserines
Polymerase Chain Reaction
Pregnancy
Pregnancy Trimester, First
Prospective Studies
Prothrombin
| | Abstract | OBJECTIVE: Antiphospholipid antibodies (APA) and other coagulation abnormalities have been associated with an increased risk of venous, arterial, and placental thrombosis and recurrent pregnancy loss (RPL). Factor V Leiden (a point mutation [1691G-->A] in the factor V gene), the prothrombin 20210G-->A mutation, and homozygosity for a common polymorphism in the methylene tetrahydrofolate reductase (MTHFR) gene (677C-->T) have been associated with arterial and venous thrombosis and arterial occlusive disease. We explored an association between these markers of thrombophilic states and RPL.
DESIGN: Prospective case-control evaluation.
SETTING: University-associated private practice. PATIENT(S): Fifty nonpregnant women with three or more pregnancy losses and 50 healthy, nonpregnant controls. INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Anticardiolipin and antiphosphatidylserine antibodies were detected in serum by ELISA. Polymerase chain reaction was performed to identify the factor V Leiden (1691G-->A) mutation, the thermobile MTHFR (677C-->T) mutation, and the prothrombin 20210G-->A mutation.
RESULT(S): The following were identified by restriction fragment-linked polymorphism analyses: 1 (2%) factor V Leiden heterozygosity; 1 (2%) prothrombin 20210G-->A heterozygosity; and 4 (8%) thermolabile MTHFR homozygosity. None of these mutation frequencies in women with RPL were statistically significantly different from controls.
CONCLUSION(S): These data suggest that factor V Leiden, thermolabile MTHFR (677C-->T), and prothrombin 20210G-->A are not found at an increased frequency in women with a history of early RPL. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 10360908 |
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