| Title | Unipolar membrane association of Dishevelled mediates Frizzled planar cell polarity signaling. | | Author(s) | Axelrod JD | | Institution | Department of Pathology, Stanford University School of Medicine, Stanford, California 94305-5324, USA. jaxelrod@cmgm.stanford.edu | | Source | Genes Dev 2001 May 15; 15(10):1182-7. | | MeSH | Animals
Blotting, Western
Cell Membrane
Cell Polarity
Drosophila
Drosophila Proteins
Green Fluorescent Proteins
Luminescent Proteins
Membrane Proteins
Microscopy, Fluorescence
Phosphoproteins
Proto-Oncogene Proteins
Receptors, Cell Surface
Repressor Proteins
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Signal Transduction
| | Abstract | Drosophila epithelia acquire a planar cell polarity (PCP) orthogonal to their apical-basal axes. Frizzled (Fz) is the receptor for the PCP signal, and Dishevelled (Dsh) transduces the signal. Here, I demonstrate that unipolar relocalization of Dsh to the membrane is required to mediate PCP, but not Wingless (Wg) signaling. Dsh membrane localization reflects the activation of Fz/PCP signaling, revealing that the initially symmetric signal evolves to one that displays unipolar asymmetry, specifying the cells' ultimate polarity. This transition from symmetric to asymmetric Dsh localization requires Dsh function, and reflects an amplification process that generates a steep intracellular activity gradient necessary to determine PCP. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 11358862 |
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