[The effect of intestinal ischemia/reperfusion on increased sensitivity to endotoxin and its potential mechanism] Zhonghua zheng xing shao shang wai ke za zhi = Zhonghua zheng xing shao shang waikf [i.e. waike] zazhi = Chinese journal of plastic surgery and burns / [Chung-hua cheng hsing shao shang wai k'o tsa chih pien chi wei yüan hui pien chi]. [Zhonghua Zheng Xing Shao Shang Wai Ke Za Zhi] Journal article | | Title | [The effect of intestinal ischemia/reperfusion on increased sensitivity to endotoxin and its potential mechanism] | | Author(s) | Yao Y, Yu Y, Chen J | | Institution | Burn Institute, 304th Hospital of People's Liberation Army, Beijing 100037. | | Source | Zhonghua Zheng Xing Shao Shang Wai Ke Za Zhi 1999 Jul; 15(4):301-4. | | MeSH | Animals
Antibodies, Monoclonal
Antigens, CD14
English Abstract
Escherichia coli
Intestine, Small
Lipopolysaccharides
Male
Multiple Organ Failure
Random Allocation
Rats
Rats, Sprague-Dawley
Reperfusion Injury
Research Support, Non-U.S. Gov't
Tumor Necrosis Factor-alpha
| | Abstract | OBJECTIVE: To investigate the effect of intestinal ischemia/reperfusion injury (I/R) on increased sensitivity to endotoxin and its potential mechanism(s).
METHODS: Sprague-Dawley rats underwent 45 minutes of superior mesenteric artery occlusion followed by reperfusion. Twelve hours after reperfusion, endotoxin (Escherichia coli LPS, 1.5 mg/kg) was injected intravenously and rats were killed 12 hours later for measurement of organ function parameters. Also, in vitro study was performed to determine LPS-induced tumor necrosis factor (TNF)release in whole blood.
RESULTS: LPS injection after I/R resulted in marked hemodynamic dysfunction and multiple organ damage(P < 0.01), while no significant or only minor changes in organ function parameters were observed in both I/R and LPS groups. In whole blood, monoclonal antibody to CD14 significantly blocked the release of TNF at low LPS concentration (< or = 10 ng/ml), and this response was dose dependent. Moreover, TNF release in LPS-stimulated whole blood obtained after I/R was inhibited to a significantly greater degree than that in baseline blood samples or sham-operation group(P < 0.05-0.01).
CONCLUSION: Splanchnic artery occlusion followed by reperfusion could lead to increased sensitivity to the subsequent LPS challenge, which may be associated with a shift toward CD14-dependent mechanism(s). | | Language | chi | | Pub Type(s) | Journal Article
| | PubMed ID | 11593613 |
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