| Title | Hormonal regulation of male-specific 20beta-hydroxysteroid dehydrogenase with carbonyl reductase-like activity present in kidney microsomes of rats. | | Author(s) | Imamura Y, Takada H, Kamizono R, Otagiri M | | Institution | Faculty of Pharmaceutical Sciences, Kumamoto University, 5-1, Oe-honmachi, 862-0973, Kumamoto, Japan. yorishig@gpo.kumamoto-u.ac.jp | | Source | J Steroid Biochem Mol Biol 2001 Oct; 78(4):373-8. | | MeSH | Alcohol Oxidoreductases
Animals
Cortisone Reductase
Female
Kidney
Male
Microsomes
Orchiectomy
Pituitary Gland
Rats
Rats, Inbred F344
Sex Factors
Substrate Specificity
| | Abstract | Progesterone, 17alpha-hydroxyprogesterone, cortisone and cortisol, which are C(21)-steroids with a ketone group at the 20-position, potently inhibited the activity of enzyme acetohexamide reductase (AHR) responsible for the reductive metabolism of acetohexamide in kidney microsomes of male rats. Furthermore, progesterone was a competitive inhibitor of AHR. In the case of progesterone usage as the substrate, 20beta-hydroxysteroid dehydrogenase (20beta-HSD) activity was much higher than 20alpha-hydroxysteroid dehydrogenase (20alpha-HSD) activity in kidney microsomes of male rats. These results indicate that AHR present in kidney microsomes of male rats, functions as 20beta-HSD with carbonyl reductase-like activity. In male rats, both testectomy and hypophysectomy decreased the renal microsomal 20beta-HSD activity, but the decreased enzyme activities were increased by the treatment with testosterone propionate (TP). We propose the possibility that TP treatment regulates the renal microsomal 20beta-HSD activity by acting directly on the kidney of male rats. This is supported from the fact that when TP was given to ovariectomized and hypophysectomized female rats, the male-specific 20beta-HSD activity was detected in their kidney microsomes. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 11717008 |
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