[Effects of Kupffer cells stimulated by triglyceride and very low-density lipoprotein on proliferation of rat hepatic stellate cells] Zhonghua nei ke za zhi [Chinese journal of internal medicine]. [Zhonghua Nei Ke Za Zhi] Journal article

OBJECTIVE: To study the effects of Kupffer cell-conditioned medium (KCCM) derived from triglyceride and very low-density lipoprotein (VLDL) treatment on proliferation of rat hepatic stellate cells (HSC).
METHODS: HSC and Kupffer cells were isolated from liver of Wistar rats by in situ perfusion with pronase and collagenase and density gradient centrifugation with Nycodenz and then cultured. KCCM was prepared and MTT colorimetric assay was used to detect HSC proliferation.
RESULTS: HSC and Kupffer cells were isolated successfully with high purity. 12.5 mg/L of triglyceride and 25 - 100 mg/L of VLDL promoted HSC proliferation (P < 0.05 or 0.01); KCCM + triglyceride and KCCM + VLDL might promote HSC proliferation as compared with a control group (P < 0.01). There was no significant difference between KCCM + triglyceride and KCCM + VLDL (P > 0.05).
CONCLUSION: The technique for isolation of HSC and Kupffer cells described here is simple and reliable. Triglyceride, VLDL and KCCM stimulated by them may promote HSC proliferation and be associated with fatty liver and hepatic fibrogenesis.

Zhonghua nei ke za zhi [Chinese journal of internal medicine]. [Zhonghua Nei Ke Za Zhi]

Animals
Cell Proliferation
Cells, Cultured
English Abstract
Hepatocytes
Kupffer Cells
Lipoproteins, VLDL
Male
Rats
Rats, Wistar
Research Support, Non-U.S. Gov't
Triglycerides