| Title | Relationship of changes in interleukin-8 levels and granulocyte elastase activity in gingival crevicular fluid to subgingival periodontopathogens following non-surgical periodontal therapy in subjects with chronic periodontitis. | | Author(s) | Jin LJ, Leung WK, Corbet EF, Söder B | | Institution | Faculty of Dentistry, University of Hong Kong, Hong Kong. ljjin@hkusua.hku.hk | | Source | J Clin Periodontol 2002 Jul; 29(7):604-14. | | MeSH | Actinobacillus actinomycetemcomitans
Adult
Alveolar Bone Loss
Bacteroides
Chronic Disease
Colony Count, Microbial
Dental Plaque
Dental Scaling
Follow-Up Studies
Gingival Crevicular Fluid
Gram-Negative Bacteria
Humans
Interleukin-8
Leukocyte Elastase
Linear Models
Matched-Pair Analysis
Middle Aged
Periodontal Attachment Loss
Periodontal Pocket
Periodontitis
Porphyromonas gingivalis
Prevotella intermedia
Research Support, Non-U.S. Gov't
Root Planing
Statistics
Statistics, Nonparametric
Treponema
| | Abstract | OBJECTIVES: To determine the effect of scaling and root planing (SRP) on the interrelations of subgingival periodontopathogens and both interleukin-8 (IL-8) and granulocyte elastase activity in gingival crevicular fluid (GCF), and to assess their relations to the short-term treatment response in management of chronic periodontitis.
MATERIAL AND METHODS: GCF and subgingival plaque were collected from 16 subjects with untreated chronic periodontitis at baseline and 4 weeks after SRP. IL-8 levels were determined by ELISA. Granulocyte elastase activity was analyzed with a specific substrate, pGluProVal-pNA, and the maximal rate of elastase activity (MR-EA) was calculated. 5 DNA-probes were used to detect the presence of A. actinomycetemcomitans (A. a.), B. forsythus (B.f.), P. gingivalis (P.g.), P. intermedia (P.i.), and T. denticola (T.d.), with a sensitivity = 103 cells/paper point.
RESULTS: IL-8 and MR-EA levels in GCF decreased significantly after SRP (p < 0.001) with a corresponding reduction of total count of the species. Of the sites with probing depth (PD) >/= 5.0 mm and co-infection by B.f., P.g., P.i. & T.d. at baseline, the sites without persistent co-infection of these species after SRP exhibited a significant reduction of IL-8 levels (p < 0.02), MR-EA levels (p < 0.02) and PD (p < 0.01). No such change was found in the sites where such a co-infection persisted. Moreover, reduction of IL-8 levels in those pocket sites was accompanied by a concomitant reduction of MR-EA (p < 0.02) and PD (p < 0.01), while no significant change in MR-EA levels and PD was noted in those pocket sites that exhibited an increase of IL-8 levels after SRP. At baseline, the former group of sites showed significantly higher IL-8 levels than the latter group of sites (p < 0.02).
CONCLUSIONS: IL-8-related granulocyte elastase activity was related to the change in infection patterns of the target periodontopathogens following scaling and root planing. Varying initial IL-8 levels in GCF and a corresponding shifting change of granulocyte elastase activity in GCF may characterize the different short-term treatment responses. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 12354085 |
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