| Title | The effect of oxidative stress on ERalpha and ERbeta expression. | | Author(s) | Tamir S, Izrael S, Vaya J | | Institution | Laboratory of Natural Medicinal Compounds, Migal-Galilee Technological Center, Kiryat Shmona 10200, Israel. | | Source | J Steroid Biochem Mol Biol 2002 Aug; 81(4-5):327-32. | | MeSH | Amidines
Antioxidants
Endothelium, Vascular
Estrogen Receptor alpha
Estrogen Receptor beta
Estrogens
Ferrous Compounds
Humans
Hydrogen Peroxide
Immunoblotting
Lipopolysaccharides
Macrophage Activation
Macrophages
Nitrogen
Oxidative Stress
Oxygen
Reactive Nitrogen Species
Reactive Oxygen Species
Receptors, Estrogen
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha
| | Abstract | The formation of intracellular reactive oxygen and nitrogen species (ROS and RNS) has been implicated in the pathogenesis of a variety of diseases. In excess, ROS and their byproducts may cause oxidative damage and be cytotoxic to cells. Recently, it has been established that these oxidants can also act as subcellular messengers in gene regulatory and signal transduction pathways. Estrogen, on the other hand, is known to offer protection from coronary artery diseases in post-menopausal women and to be involved in various ROS-related diseases, such as Alzheimer's and Parkinson's diseases, diabetes and aging. The existence of estrogen receptors in these tissues lead us to investigate whether ROS can regulate their expression. We demonstrated here, for the first time, that oxidative stress induced by hydrogen peroxide (H(2)O(2)), Fe(2+), 2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH) and activated macrophages, affect the expression of estrogen receptors alpha and beta (ERalpha and ERbeta) differently, demonstrating cell-specific response which can be blocked by antioxidants. This data suggest that oxidative stress and the production of ROS/RNS function as physiological regulators of ERalpha and ERbeta expression. This may provide a new insight into the ERbeta-dependent protective action of estrogen and phytoestrogens in inflammation involving diseases, and may contribute to the development of novel therapeutic treatment strategies. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 12361722 |
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