| Title | Development of a mucosal complex vaccine against oral Salmonella infection in mice. | | Author(s) | Harada H, Nishikawa F, Higashi N, Kita E | | Institution | Department of Bacteriology, Nara Medical University, Kashihara, Nara 634-8521, Japan. | | Source | Microbiol Immunol 2002; 46(12):891-905. | | MeSH | Administration, Oral
Animals
CD4-Positive T-Lymphocytes
Cholera Toxin
Cytokines
Female
Flagellin
Hypersensitivity, Delayed
Immunoglobulin A
Immunoglobulin G
Immunoglobulin M
Intestines
Liver
Mice
Mice, Inbred C57BL
Mouth Mucosa
RNA, Messenger
Research Design
Salmonella Infections
Salmonella Vaccines
Salmonella typhimurium
Spleen
Th1 Cells
| | Abstract | We examined the immunogenicity of a Salmonella enterica complex vaccine (CV), consisting of flagellin and polysome purified from serotype Typhimurium LT2. CV plus cholera toxin (CT), in three oral doses given at 7-day intervals, conferred complete protection on C57BL/6 mice against lethal oral infection with a wild-type strain. It elicited mucosal IgA > IgG2a > IgG1 and systemic IgG2a > IgG1 > IgA antibodies to flagellin and polysome, and delayed footpad response (DFR) to both antigens. In Peyer's patches (PPs) and lamina propria (LP), IgA was produced under a Th1-dominant environment; CD4+T cells from produced interleukin (IL)-2, interferon (IFN)-gamma, and IL-10 by stimulation with salmonella extract. On the same protocol, flagellin plus CT induced flagellin-specific mucosal and systemic IgA and IgG1 antibodies, CD4+T cells producing IL-10 and IFN-gamma in PPs and LP, and only minimal levels of flagellin-specific DFR. Polysome plus CT induced polysome-specific mucosal and systemic IgG2a in addition to IgG1 and IgA antibodies, CD4+T cells producing IFN-gamma and IL-2 in PPs and LP, and polysome-specific DFR. These two vaccines, however, conferred at most 50-60% survival rates. Our results suggest that polysomes in CV provide effective adjuvant activity for the induction of both mucosal and systemic Th1-biased responses toward flagellin. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 12597365 |
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