| Title | Werner's syndrome protein is phosphorylated in an ATR/ATM-dependent manner following replication arrest and DNA damage induced during the S phase of the cell cycle. | | Author(s) | Pichierri P, Rosselli F, Franchitto A | | Institution | CNRS, UPR2169 'Genetic Instability and Cancer', Institut Gustave Roussy, France. | | Source | Oncogene 2003 Mar 13; 22(10):1491-500. | | MeSH | Camptothecin
Cell Cycle Proteins
Cell Nucleus Structures
Cells, Cultured
DNA Damage
DNA Helicases
DNA Replication
DNA-Binding Proteins
Enzyme Inhibitors
Fibroblasts
Humans
Hydroxyurea
Lymphocytes
Phosphorylation
Protein-Serine-Threonine Kinases
Research Support, Non-U.S. Gov't
S Phase
Tumor Suppressor Proteins
Werner Syndrome
| | Abstract | Werner's syndrome (WS) is an autosomal recessive disorder, characterized at the cellular level by genomic instability in the form of variegated translocation mosaicism and extensive deletions. Individuals with WS prematurely develop multiple age-related pathologies and exhibit increased incidence of cancer. WRN, the gene defective in WS, encodes a 160-kDa protein (WRN), which has 3'-5'exonuclease, DNA helicase and DNA-dependent ATPase activities. WRN-defective cells are hypersensitive to certain genotoxic agents that cause replication arrest and/or double-strand breaks at the replication fork, suggesting a pivotal role for WRN in the protection of the integrity of the genoma during the DNA replication process. Here, we show that WRN is phosphorylated through an ATR/ATM dependent pathway in response to replication blockage. However, we provide evidence that WRN phosphorylation is not essential for its subnuclear relocalization after replication arrest. Finally, we show that WRN and ATR colocalize after replication fork arrest, suggesting that WRN and the ATR kinase collaborate to prevent genome instability during the S phase. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 12629512 |
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