| Title | Sex differences in in vitro pro-inflammatory cytokine production from peripheral blood of multiple sclerosis patients. | | Author(s) | Nguyen LT, Ramanathan M, Weinstock-Guttman B, Baier M, Brownscheidle C, Jacobs LD | | Institution | Department of Pharmaceutical Sciences, State University of New York at Buffalo, 543 Cooke Hall, Buffalo, NY 14260-1200, USA. | | Source | J Neurol Sci 2003 May 15; 209(1-2):93-9. | | MeSH | Adolescent
Adult
Aged
Analysis of Variance
Antigens, CD3
Antigens, CD5
Cytokines
Disability Evaluation
Female
Humans
Inflammation Mediators
Interferon Type II
Interleukin-10
Interleukin-2
Interleukin-4
Male
Middle Aged
Multiple Sclerosis, Chronic Progressive
Multiple Sclerosis, Relapsing-Remitting
Research Support, U.S. Gov't, P.H.S.
Sex Factors
T-Lymphocytes
Transforming Growth Factor beta
Tumor Necrosis Factor-alpha
| | Abstract | We compared the patterns of the pro-inflammatory cytokines, interferon-gamma (IFN-gamma), interleukin-2 (IL-2) and tumor necrosis factor-alpha (TNF-alpha), and the anti-inflammatory cytokines, interleukin-10 (IL-10) and tumor growth factor-beta (TGF-beta) from peripheral blood of male and female patients with relapsing-remitting (RR) and secondary progressive (SP) forms of multiple sclerosis (MS). The relationships between pro-inflammatory cytokines and disability (expanded disability status scale, EDSS) were also examined.Peripheral blood anti-coagulated with heparin was obtained from 47 MS patients (30 women and 17 men) and activated with phorbol-12-myristate 13 acetate (PMA) and ionomycin in the presence of brefeldin A and stained for flow cytometry with fluorescently labeled antibodies against intracellular IFN-gamma, TNF-alpha, IL-2, IL-4 and IL-10. The T cells were delineated with peridinin chlorophyll protein (Per-CP) labeled anti-CD3 antibody. The stained samples were analyzed on a flow cytometer to assess the intracellular pro-inflammatory cytokine patterns. The levels of interleukin-10 (IL-10) and tumor growth factor-beta (TGF-beta) were measured in plasma using enzyme-linked immunoassay.The percentage of TNF-alpha-producing CD3 positive cells was significantly higher (P=0.045) in men (mean+/-S.D., 39+/-13%) than in women (mean+/-S.D., 29+/-13%) RR-MS patients. The percentage of CD3 positive cells producing IFN-gamma was significantly correlated with EDSS in females but not in males (Spearman rank correlation r(S)=0.49, P=0.018). The secretion of the pro-inflammatory cytokines, IFN-gamma and TNF-alpha, is influenced by gender in MS patients and may contribute to the sexual dimorphism of MS. | | Language | eng | | Pub Type(s) | Clinical Trial
Controlled Clinical Trial
Journal Article
| | PubMed ID | 12686409 |
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