| Title | Polymorphism at position -174 of IL-6 gene is associated with susceptibility to chronic periodontitis in a Caucasian Brazilian population. | | Author(s) | Trevilatto PC, Scarel-Caminaga RM, de Brito RB, de Souza AP, Line SR | | Institution | Department of Morphology, Dental School of Piracicaba, State University of Campinas, SP, Brazil. | | Source | J Clin Periodontol 2003 May; 30(5):438-42. | | MeSH | Adult
Alleles
Base Sequence
Brazil
Chi-Square Distribution
Chronic Disease
Cytosine
European Continental Ancestry Group
Female
Gene Frequency
Genetic Predisposition to Disease
Genotype
Guanine
Humans
Inflammation Mediators
Interleukin-6
Male
Periodontitis
Polymorphism, Genetic
Promoter Regions (Genetics)
Research Support, Non-U.S. Gov't
Transcription, Genetic
| | Abstract | BACKGROUND: Interleukin-6 (IL-6) is a multifunctional cytokine that mediates inflammatory tissue destruction. A G to C substitution at position -174 in the promoter of IL-6 gene reduces in vitro transcription of IL-6. This polymorphism has been associated with inflammatory diseases like chronic arthritis.
OBJECTIVE: The aim of this study was to investigate the association between the IL-6-174 polymorphism and susceptibility to chronic periodontitis in Brazilians.
MATERIAL AND METHODS: Eighty-four nonsmoking subjects over 25 years (mean age 42.4) were divided according to the severity level of periodontal disease: 36 healthy individuals (control group), 24 subjects with moderate and 24 with severe periodontitis. Genomic DNA was obtained from epithelial cells through a mouthwash with 3% glucose and scraping of oral mucosa. The samples were analyzed for IL-6-174 polymorphism using PCR-RFLP. The significance of the differences in the frequencies of the polymorphism in the control and groups with periodontitis was assessed by chi2 test (p<0.05).
RESULTS: Differences were found between control and groups with periodontitis in the genotype (p=0.0036, OR=3.0) and in the allele (p=0.0838, OR=1.9) frequencies.
CONCLUSION: We concluded that the IL-6-174 polymorphism is associated with susceptibility to chronic periodontitis in the population studied. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 12716337 |
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