| Title | Cytokine microenvironments in human first trimester decidua are dependent on trophoblast cells. | | Author(s) | von Rango U, Classen-Linke I, Raven G, Bocken F, Beier HM | | Institution | Department of Anatomy and Reproductive Biology, School of Medicine, RWTH University of Aachen, Aachen, Germany. uvonrango@ukaachen.de | | Source | Fertil Steril 2003 May; 79(5):1176-86. | | MeSH | Cytokines
Decidua
Female
Humans
Immunohistochemistry
Pregnancy
Pregnancy Trimester, First
RNA, Messenger
Research Support, Non-U.S. Gov't
Retrospective Studies
Reverse Transcriptase Polymerase Chain Reaction
Trophoblasts
| | Abstract | OBJECTIVE: To compare cytokine expression profiles of decidua basalis (containing trophoblast cells) and decidua parietalis (without trophoblast cells) for determination of microenvironments in human first trimester decidua.
DESIGN: Retrospective study.
SETTING: School of Medicine, RWTH University of Aachen, Aachen Germany, and Bourgognekliniek Maastricht, Maastricht, The Netherlands. PATIENT(S): Forty-six women who had undergone elective first-trimester termination of viable pregnancy at 5 to 12 weeks.
MAIN OUTCOME MEASURE(S): Quantitative cytokine protein analysis in decidual tissues by enzyme-linked immunosorbent assay, qualitative cytokine messenger (m)RNA analysis in isolated decidual cell samples, and comparative mRNA and protein analysis in tissues of decidua basalis compared with decidua parietalis.
RESULT(S): Interleukin-2, interferon-gamma (Th-1), interleukin-4 (Th-2), and interleukin-1beta proteins are expressed in the human first-trimester decidua. Interleukin-2, interferon-gamma, and interleukin-4 mRNA mainly derive from the decidual tissue leukocytes. Interleukin-1beta mRNA is expressed by all decidual cell types. Interferon-gamma mRNA and protein is detected predominantly in the decidua basalis, which contains trophoblast cells.
CONCLUSION(S): Microenvironments are established topographically by different expression of cytokines in decidua basalis and decidua parietalis. These locally specific patterns are indicative of fetomaternal cross-talk. Higher interferon-gamma concentrations in decidua basalis may influence leukocyte differentiation (e.g., macrophage activation) and trophoblast invasion (e.g., by induction of expression of major histocompatibility complex). | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 12738514 |
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