Effects of the interaction between the C677T 5,10-methylenetetrahydrofolate reductase polymorphism and serum B vitamins on homocysteine levels in pregnant women. European journal of clinical nutrition. [Eur J Clin Nutr] Journal article | | Title | Effects of the interaction between the C677T 5,10-methylenetetrahydrofolate reductase polymorphism and serum B vitamins on homocysteine levels in pregnant women. | | Author(s) | Kim KN, Kim YJ, Chang N | | Institution | Department of Food and Nutritional Sciences, Ewha Womans University, Seoul, Korea. | | Source | Eur J Clin Nutr 2004 Jan; 58(1):10-6. | | MeSH | Adult
Chromatography, High Pressure Liquid
Cross-Sectional Studies
Female
Folic Acid
Genotype
Homocysteine
Humans
Methylenetetrahydrofolate Reductase (NADPH2)
Mutation
Polymerase Chain Reaction
Polymorphism, Genetic
Pregnancy
Radioimmunoassay
Research Support, Non-U.S. Gov't
Riboflavin
Vitamin B 12
Vitamin B 6
| | Abstract | OBJECTIVE: The purpose of this study was to investigate the effect of the interaction between the C677T mutation in the 5,10-methylenetetrahydrofolate reductase (MTHFR) genotypes and serum levels of B vitamins on serum homocysteine levels in pregnant women.
DESIGN: A cross-sectional study.
SETTING: Ewha Womans University Hospital, Seoul, Korea.
SUBJECTS: A total of 177 normal pregnant women, 24.6+/-1.1 weeks of gestation, in a 6-month period during 2001-2002.
INTERVENTIONS: Serum vitamin B2, vitamin B6, and homocysteine analyses were conducted using high-performance liquid chromatography methods. Serum folate and vitamin B12 concentrations were determined using a radioimmunoassay kit. MTHFR gene mutation was investigated by the polymerase chain reaction of a genomic DNA fragment.
RESULTS: Serum homocysteine was higher in women with the T/T genotype than those with the C/T or C/C genotype of the MTHFR gene (P<0.05). Serum homocysteine was negatively correlated with serum folate in all MTHFR genotypes (P<0.001), and the correlation between the two serum levels was the strongest in the T/T genotype. Serum homocysteine was higher in the subjects with the T/T MTHFR genotype only when the serum folate was below the median level. Explanatory power of B vitamin status as predictors of serum homocysteine levels was more pronounced in the T/T genotypes (68.5%) compared with the C/T (37.9%) or C/C genotypes (20.6%).
CONCLUSIONS: Serum homocysteine levels in pregnant women varied significantly with MTHFR genotype and the serum B vitamin status. Higher serum folate, vitamin B2, and vitamin B12 concentrations may lessen the MTHFR genotypic effect on serum homocysteine levels. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 14679361 |
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