| Title | Endothelial cell injury and platelet aggregation induced by contrast ultrasonography in the rat hepatic sinusoid. | | Author(s) | Shigeta K, Itoh K, Ookawara S, Taniguchi N, Omoto K | | Institution | Department of Clinical Laboratory Medicine, Jichi Medical School, Kawachi-gun, Tochigi-ken, Japan. shigeta@jichi.ac.jp | | Source | J Ultrasound Med 2004 Jan; 23(1):29-36. | | MeSH | Animals
Contrast Media
Endothelium
Liver
Liver Diseases
Male
Microscopy, Electron
Platelet Aggregation
Polysaccharides
Rats
Rats, Wistar
| | Abstract | OBJECTIVE: To determine whether contrast ultrasonography can affect the sinusoidal cells and platelets of the liver by using ultrastructural analysis in vivo.
METHODS: Fifteen Wistar rats were placed into the following 5 groups of 3 rats each: 3 control groups comprising a sham operation group, a contrast agent injection-alone group, and an ultrasound exposure-alone group; and 2 contrast agent injection with ultrasound exposure groups, split according to excision time. After a dose of an echo contrast agent (100 mg/kg of body weight) was administered through the femoral vein, the rats that received injections were subjected to ultrasound for the first minute, no ultrasound for the next 4 minutes, and then ultrasound sweep scanning for 10 seconds. The rats were perfused via the heart with cold physiologic saline containing 2% paraformaldehyde and 2.5% glutaraldehyde solution buffered with 0.1-mol/L phosphate. The livers of the rats in 4 of the groups were excised immediately. The livers of the rats in 1 of the 2 contrast agent with ultrasound exposure groups were excised by the same procedure 5 hours after they received the injections. All specimens were studied with light and electron microscopy.
RESULTS: Platelet aggregation and injury to endothelial cells were more severe in the contrast agent injection and ultrasound exposure groups than in the other groups.
CONCLUSIONS: Contrast ultrasonography can cause platelet aggregation and endothelial cell damage in the rat hepatic sinusoid. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 14756350 |
|
