| Title | Photodynamic therapy of intestinal tumors with mono-L-aspartyl chlorin e6 (NPe6): a basic study. | | Author(s) | Kikuchi T, Asakura T, Aihara H, Shiraki M, Takagi S, Kinouchi Y, Aizawa K, Shimosegawa T | | Institution | Division of Gastroenterology, Tohoku University Graduate School of Medicine, 1-1 Seiryo, Aoba, Sendai 980-8574, Japan. dr_tatsu@pg.highway.ne.jp | | Source | Anticancer Res 2003 Nov-Dec; 23(6C):4897-900. | | MeSH | Animals
Disease Models, Animal
Intestinal Neoplasms
Lasers
Mice
Mice, Inbred C57BL
Mice, Inbred Strains
Microscopy, Fluorescence
Photochemotherapy
Photosensitizing Agents
Porphyrins
| | Abstract | BACKGROUND: To clarify the usefulness of the second-generation photosensitizer, mono-L-aspartyl chlorin e6 (NPe6), we examined the possibility of photodynamic diagnosis and photodynamic therapy for intestinal tumors in a mouse model.
MATERIALS AND METHODS: NPe6 was intravenously administered to the tumor-bearing mice through the tail vein. The intestinal tumor sites were irradiated with a 664-nm diode laser at constant intervals after the administration of photosensitizers. The tumors were excised and fluorescence was observed in frozen sections by microscope.
RESULTS: We observed the fluorescent image and calculated that the mean fluorescence intensity was significantly higher in the tumors than the normal mucosa during 6 hours (p < 0.05). The fluorescence of NPe6 was chiefly accumulated in the intestinal tumors as red fluorescence on the fluorescent microphotographic image.
CONCLUSION: We conclude that NPe6 may be a valuable photosensitizer for the photodynamic diagnosis and photodynamic therapy of intestinal tumors. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 14981942 |
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