| Title | Locally produced anti-phosphorylcholine and anti-oxidized low-density lipoprotein antibodies in gingival crevicular fluid from aggressive periodontitis patients. | | Author(s) | Schenkein HA, Berry CR, Burmeister JA, Brooks CN, Best AM, Tew JG | | Institution | Virginia Commonwealth University School of Dentistry, Clinical Research Center for Periodontal Disease, Richmond, VA 23298-0566, USA. haschenk@vcu.edu | | Source | J Periodontol 2004 Jan; 75(1):146-53. | | MeSH | Acute Disease
Adult
Analysis of Variance
Antibodies, Bacterial
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Female
Gingival Crevicular Fluid
Humans
Immunoglobulin G
Lipoproteins, LDL
Male
Periodontitis
Phosphorylcholine
Serum Albumin
Tetanus Toxoid
| | Abstract | BACKGROUND: Sera from patients with periodontal attachment loss contain higher concentrations of IgG anti-phosphorylcholine (anti-PC) than sera from healthy subjects. Furthermore, a large proportion of plaque bacteria bear PC-containing surface antigens, implicating the oral flora as a source of immunogen for anti-PC. Additionally, anti-PC is cross-reactive with a variety of oral bacterial antigens and human antigens such as oxidized low-density lipoprotein (oxLDL). We hypothesized that, if the oral flora is a source of PC antigens, then we should be able to detect local anti-PC and anti-oxLDL production in gingival crevicular fluid (GCF).
METHODS: To test this, we collected 66 GCF samples from 15 patients with aggressive periodontitis and examined both the GCF samples and serum samples for their content of IgG anti-PC, IgG anti-LDL, and IgG anti-oxLDL by enzyme-linked immunosorbent assay. We also determined levels of anti-tetanus toxoid (anti-TT) as a non-oral antigen control. Serum and GCF concentrations of serum albumin (HSA) were also determined for use as a dilution marker. A conservative GCF:serum antibody ratio of greater than 1.5 was considered to be evidence of local antibody production.
RESULTS: For the non-oral antigen TT, only one out of 62 samples contained locally produced antibody. Eight out of 64 samples (7 from a single subject) demonstrated local production of anti-LDL. In contrast, 28 out of 66 samples demonstrated local production of anti-PC, and 47 out of 66 samples contained locally produced anti-oxLDL. It was observed that A. actinomycetemcomitans strains containing or devoid of PC could absorb anti-oxLDL from human sera. Although there was a correlation between the ratios of anti-PC and anti-oxLDL (Spearman's rho = 0.35, P = 0.0037), local production of both antibodies was found in only 17 out of 65 samples, indicating that these antibodies are not always reflective of reactivity to the same antigens.
CONCLUSION: The local production of anti-PC and anti-oxLDL further implicates the oral flora as a source of antigen that may mediate immune reactions of relevance to cardiovascular and other systemic diseases. | | Language | eng | | Pub Type(s) | Journal Article
Research Support, U.S. Gov't, P.H.S.
| | PubMed ID | 15025226 |
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