| Title | Immunogenic properties of the Salmonella atypical fimbriae in BALB/c mice. | | Author(s) | Strindelius L, Folkesson A, Normark S, Sjöholm I | | Institution | Department of Pharmacy, Biomedical Centre, Uppsala University, Box 580, Uppsala SE-751 23, Sweden. | | Source | Vaccine 2004 Mar 29; 22(11-12):1448-56. | | MeSH | Adjuvants, Immunologic
Animals
Antibodies, Bacterial
Antibody Formation
Cholera Toxin
DNA Primers
Drug Delivery Systems
Enzyme-Linked Immunosorbent Assay
Feces
Female
Fimbriae, Bacterial
Immunity, Mucosal
Immunoglobulin A
Immunoglobulin G
Immunoglobulin M
Mice
Mice, Inbred BALB C
Research Support, Non-U.S. Gov't
Reverse Transcriptase Polymerase Chain Reaction
Salmonella
Salmonella Infections
Salmonella Vaccines
Salmonella enteritidis
Starch
Vaccines, Conjugate
Vaccines, Synthetic
| | Abstract | Components of the Salmonella atypical fimbriae (Saf) were investigated for potential inclusion in a Salmonella vaccine. Recombinant histidine-tagged SafB chaperone complexed with SafD adhesin was expressed in Escherichia coli and purified. Starch microparticles were used, as an adjuvant and recombinant cholera toxin B subunit (rCTB) was included as a mucosal antigen-uptake enhancer. BALB/c mice were immunized orally or subcutaneously with SafB/D- and rCTB-conjugated microparticles and nasally or subcutaneously with SafB/D mixed with rCTB. The systemic and mucosal immune responses were studied, and an oral challenge with Salmonella enteritidis was performed. All immunized groups except that receiving oral immunization responded with high IgM-IgG titers to SafB/D. Analysis of the subclass ratio (IgG1/IgG2a+IgG2b) indicated a mixed Th1 and Th2 response, with Th1 predominating. The mucosal response, measured as specific IgA/total IgA (from fecal samples), was significantly greater than that in the untreated control group only in the group receiving intranasal immunization (P<0.05). Spleens were removed 6 days after oral challenge and Salmonella colony-forming units (CFU) were counted. The group immunized subcutaneously with SafB/D- and rCTB-conjugated microparticles had significantly lower CFU counts than the untreated control group (P<0.05). | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 15063568 |
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