Expression of leukemia inhibitory factor and its receptor is not altered in the decidua and chorionic villi of human anembryonic pregnancy. Human reproduction (Oxford, England) [Hum Reprod] Journal article | | Title | Expression of leukemia inhibitory factor and its receptor is not altered in the decidua and chorionic villi of human anembryonic pregnancy. | | Author(s) | Chen HF, Chao KH, Shew JY, Yang YS, Ho HN | | Institution | Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University. | | Source | Hum Reprod 2004 Jul; 19(7):1647-54. | | MeSH | Adult
Case-Control Studies
Chorionic Villi
Computer Systems
Decidua
Female
Gene Expression
Humans
Immunohistochemistry
Interleukin-6
Polymerase Chain Reaction
Pregnancy
Pregnancy Complications
RNA, Messenger
Receptors, Cytokine
Research Support, Non-U.S. Gov't
| | Abstract | BACKGROUND: Uterine expression of leukemia inhibitory factor (LIF) is absolutely essential for mouse, and critical for human, embryo implantation. However LIF is not required for post-implantation development of mouse embryo. The objective of this study was to examine the role of LIF system in post-implantation stage of human pregnancy.
METHODS: Tissues from 25 patients with anembryonic pregnancy (AP; blighted ovum) and 25 matched patients with normal pregnancy (NP) were collected. LIF and its receptor beta (LIF-Rbeta) expression in the decidua and chorionic villi were analyzed by semi-quantitative reverse transcription and polymerase chain reaction (RT-PCR), real-time quantitative PCR and immunohistochemical study.
RESULTS: LIF mRNA levels were not different either between different tissues (decidua vs chorionic villi) or between different patients (NP vs AP). LIF-Rbeta mRNA levels were significantly higher in chorionic villi than in decidua but were not different between NP and AP. Immunohistochemical staining supported these findings and showed a predominate expression of LIF-Rbeta in the trophoblast cells.
CONCLUSIONS: This study concluded that at early human post-implantation stage, LIF is produced from both decidua and chorionic villi and may exert its major action on trophoblasts. A baseline expression of LIF and LIF-Rbeta is probably needed for early pregnancy, but AP cannot be accounted for by the defective expression of either LIF or LIF-Rbeta in most circumstances. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 15180980 |
|
|
| |
Advertise on this site.
| | |
|
