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Comparison of viability of B16 and Cl S91 cells in three cytotoxicity tests: cells counting, MTT and flow cytometry after cytostatic drug treatment. Acta poloniae pharmaceutica. [Acta Pol Pharm] Journal article

 
Olszewska-Słonina D, Drewa T, Musiałkiewicz D, Olszewski K 
Comparison of viability of B16 and Cl S91 cells in three cytotoxicity tests: cells counting, MTT and flow cytometry after cytostatic drug treatment. [Journal Article]
Acta Pol Pharm 2004 Jan-Feb; 61(1):31-7.


In this study, results of three tests assessing viability of B16 and Cl S91 mouse melanoma cells after exposure to cytostatic drugs were compared: alive cell counting test after fixing with ethyl alcohol, MTT test and annexin V-FITC flow cytometry test. On the grounds of the obtained results the last mentioned method was recognized to be the most accurate and the least faulty. It was stated that mouse melanoma B16 cells are more sensitive to actinomycin D. cytosine arabinoside, cisplatin and dacarbazine than mouse melanoma Cl S91 cells. Mouse melanoma Cl S91 cells are more sensitive to vincristine than B16 cells.



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