Comparison of viability of B16 and Cl S91 cells in three cytotoxicity tests: cells counting, MTT and flow cytometry after cytostatic drug treatment. Acta poloniae pharmaceutica. [Acta Pol Pharm] Journal article | | Title | Comparison of viability of B16 and Cl S91 cells in three cytotoxicity tests: cells counting, MTT and flow cytometry after cytostatic drug treatment. | | Author(s) | Olszewska-Słonina D, Drewa T, Musiałkiewicz D, Olszewski K | | Institution | Department of Medical Biology, 2 Department and Clinic of Orthopedics and Traumathology, Rydygier Medical University, 24 Karlowicza Str., 85-092 Bydgoszcz, Poland. | | Source | Acta Pol Pharm 2004 Jan-Feb; 61(1):31-7. | | MeSH | Animals
Annexin A5
Antineoplastic Agents
Apoptosis
Cell Count
Cell Line, Tumor
Cell Survival
Colorimetry
Coloring Agents
Comparative Study
Drug Evaluation, Preclinical
Flow Cytometry
Fluorescein-5-isothiocyanate
Fluorescent Dyes
Melanoma, Experimental
Mice
Mice, Inbred C57BL
Necrosis
Propidium
Tetrazolium Salts
Thiazoles
| | Abstract | In this study, results of three tests assessing viability of B16 and Cl S91 mouse melanoma cells after exposure to cytostatic drugs were compared: alive cell counting test after fixing with ethyl alcohol, MTT test and annexin V-FITC flow cytometry test. On the grounds of the obtained results the last mentioned method was recognized to be the most accurate and the least faulty. It was stated that mouse melanoma B16 cells are more sensitive to actinomycin D. cytosine arabinoside, cisplatin and dacarbazine than mouse melanoma Cl S91 cells. Mouse melanoma Cl S91 cells are more sensitive to vincristine than B16 cells. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 15259855 |
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