| Title | [Clinical application of the activated partial thromboplastin time (APTT) with modified incubation time for the diagnosis of lupus anticoagulant (LA)] | | Author(s) | Fardella P, Rustagi PK | | Institution | Servicio de Hematología, Hospital Clinico Universidad de Chile, Santiago, Chile. pfardella123@msn.com | | Source | Rev Med Chil 2004 Apr; 132(4):407-12. | | MeSH | Adult
Anticoagulants
Antiphospholipid Syndrome
Case-Control Studies
English Abstract
False Positive Reactions
Female
Heparin
Humans
Lupus Coagulation Inhibitor
Partial Thromboplastin Time
Pregnancy
Pregnancy Complications
Prothrombin Time
Sensitivity and Specificity
Thrombin Time
Warfarin
| | Abstract | BACKGROUND: Although several tests are used to screen for the presence of LA, none detects all its types. The shortening of APT observed when the pre-incubation period is prolonged, proved to be a sensitive test for the presence of LA.
MATERIAL AND METHODS: We determined the APTT, performed with a 4 or 15 min preincubation period (APETs and APTT15 respectively), in 22 healthy subjects, 3 commercial positive controls for LA, 16 patients with a previous diagnosis of LA and 54 patients with recurrent fetal loss and/or infertility. Evidence of LA was established by a positive Staclot-LA test.
RESULTS: APTTs and APTT15 were 31.5 +/- 4.7 and 28.4 +/- 4.5 seconds respectively in samples from 22 normal subjects. The figures in samples with LA, were 71.5 +/- 20.3 s and 58.6 +/- 18 s respectively. The difference between the two APTTs performed on an individual sample was defined as the APTT 4-15 and was 2.6 +/- 2.0 in normal subjects 2.5 +/- 2.8 in 13 patients anticoagulated with warfarin, -10.0 +/- 6.5 in 13 patients receiving heparin, and 13.2 +/- 4.9 in 15 patients with LA. The test values for LA patients were significantly higher than those for normal subjects (p < 0.0001). For values over 5, the APTT 4-15 had 93.3% sensitivity and 100% specificity. In one patient with recurrent fetal loss or infertility, who was LA positive, the APTT 4-15 was positive with a value of 14.
CONCLUSIONS: This modified TTPA is easy to perform, and provides a reasonably discriminatory value for the presence of LA. Therefore, we recommend the TTPA 4-15 to screen for LA. | | Language | spa | | Pub Type(s) | Evaluation Studies
Journal Article
| | PubMed ID | 15382511 |
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