Performance of the immunoglobulin G avidity and enzyme immunoassay IgG/IgM screening tests for differentiation of the clinical spectrum of toxoplasmosis. Journal of microbiology (Seoul, Korea) [J Microbiol] Journal article

Title	Performance of the immunoglobulin G avidity and enzyme immunoassay IgG/IgM screening tests for differentiation of the clinical spectrum of toxoplasmosis.
Author(s)	Tanyuksel M, Guney C, Araz E, Saracli MA, Doganci L
Institution	Division of Medical Parasitology, Department of Microbiology and Clinical Microbiology,Gulhane Military Medical Academy, Ankara, Turkey. mtanyuksel@gata.edu.tr
Source	J Microbiol 2004 Sep; 42(3):211-5.
MeSH	Animals Antibodies, Protozoan Antibody Affinity Chorioretinitis Comparative Study Enzyme-Linked Immunosorbent Assay Female Humans Immunoenzyme Techniques Immunoglobulin G Immunoglobulin M Male Pregnancy Pregnancy Complications, Parasitic Pregnancy Trimester, First Reagent Kits, Diagnostic Toxoplasma Toxoplasmosis, Ocular
Abstract	Toxoplasmosis has been well known as an important human infection to consider especially in pregnant women. Although many serologic methods are available, the diagnosis of toxoplasmosis can be extremely difficult. The presence of increased levels of Toxoplasma-specific IgG antibodies indicates an infection, but it does not differentiate between a recent and past infection. The purpose of our study was to compare the performance of the ELISA T. gondii IgG/IgM test, a widely used enzyme-linked immunosorbent assay, to the ELISA IgG avidity method. One hundred and four serum samples (from 38 males and 66 females) were tested and evaluated from symptomatic patients (chorioretinitis, lymphadenopathy), and from women in their first trimester of pregnancy who were suspected of having toxoplasmosis. The high IgG avidity and ELISA IgG antibody levels were in agreement for 51 of the specimens (49.0%). Thirty-eight discrepant (borderline) results from the IgG avidity method were positive for IgM (3 specimens) and IgG (37 specimens). Interestingly, out of the eight serum samples that were positive for both IgG and IgM antibodies, two samples were low IgG avidity, and three samples were borderline. There was no statistically significant relation observed between the results of the IgG avidity method and the ELISA IgG test, and the IgG avidity method and ELISA IgM test (chi2 = 1.987; p = 0.370 and chi2 = 2.152; p = 0.341, respectively). The IgG avidity method was considered easy to perform and an acceptable approach for the differentiation of discrepant results (recent/chronic) and for the current detection of T. gondii antibodies. We concluded that the determination of IgG avidity is a helpful tool for the diagnosis of the ocular form of toxoplasmosis and it is a safe method for screening this disease in the first trimester of pregnancy.
Language	eng
Pub Type(s)	Evaluation Studies Journal Article
PubMed ID	15459650

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