| Title | Comparison of the analysis of corticosteroids using different techniques. | | Author(s) | Pujos E, Flament-Waton MM, Paisse O, Grenier-Loustalot MF | | Institution | Service Central d'Analyses, USR 059, CNRS, BP 22, Vernaison 69390, France. | | Source | Anal Bioanal Chem 2005 Jan; 381(1):244-54. | | MeSH | Adrenal Cortex Hormones
Chromatography, Liquid
Comparative Study
Enzyme-Linked Immunosorbent Assay
Humans
Mass Fragmentography
Reference Standards
Spectrometry, Mass, Electrospray Ionization
Spectrophotometry, Ultraviolet
| | Abstract | In this work we have optimized the analysis of 18 human corticosteroids, some endogenous (tetrahydrocortisol, tetrahydrocortisone, cortisol, and cortisone) and others synthetic (betamethasone, budesonide, cortisone acetate, desonide, dexamethasone, dexamethasone acetate, flunisolide, fluocinolone acetonide, halcinonide, methylprednisolone, prednisolone, prednisone, triamcinolone, and triamcinolone acetonide). Three analytical techniques were developed: ELISA, gas chromatography coupled with mass spectrometry (GC-MS), and liquid chromatography coupled with mass spectrometry (LC-MS). Several sample-preparation methods were optimized for each technique and enabled compounds of interest to be extracted from small urine samples (several mL). The results enabled us to assess the possibilities and the sensitivity of each technique for application to doping tests. | | Language | eng | | Pub Type(s) | Journal Article
Validation Studies
| | PubMed ID | 15700162 |
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