| Title | Increasing storage time of extended boar semen reduces sperm DNA integrity. | | Author(s) | Boe-Hansen GB, Ersbøll AK, Greve T, Christensen P | | Institution | Department of Large Animal Sciences, Veterinary Reproduction and Obstetrics, The Royal Veterinary and Agricultural University, Dyrlaegevej 68, 1870 Frederiksberg C, Denmark. gbh@kvl.dk | | Source | Theriogenology 2005 Apr 15; 63(7):2006-19. | | MeSH | Acridine Orange
Animals
Chromatin
Cryopreservation
DNA
Flow Cytometry
Fluorescent Dyes
Male
Organic Chemicals
Research Support, Non-U.S. Gov't
Semen
Semen Preservation
Sperm Count
Spermatozoa
Statistics, Nonparametric
Swine
| | Abstract | There is an extensive use of artificial insemination (AI) in the pig industry. Extended liquid boar semen may be used for insemination for up to 5 days after collection. The objective of this study was to determine the changes in sperm quality, when boar semen was extended and stored at 18 degrees C for up to 72 h post-collection. The study included three ejaculates from five boars, for each of the four breeds: Duroc, Hampshire, Landrace and Danish Large White (n=60 ejaculates). The sperm chromatin structure assay (SCSA) showed an increase in DNA fragmentation index (DFI) after 72 h of incubation (P<0.001), with no differences between breeds (P=0.07). For two Hampshire boars, all ejaculates had a large increase in DFI after 24 h of incubation. The standard deviation of DFI (SD-DFI) differed between breeds, with the SD-DFI for Hampshire being significantly greater than for the other breeds. The SD-DFI did not change during the 72 h of storage. Sperm viability was determined using SYBR-14 and propidium iodide in combination with flow cytometry. The sperm viability did not differ between breeds (P=0.21), but a difference in viability during storage (P<0.001) was detected. In conclusion, the SCSA cytogram patterns were consistent for different ejaculates within boars and storage of extended boar semen at 18 degrees C for 72 h significantly decreased the integrity of sperm DNA. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 15823356 |
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