| Title | Effect of ProRoot MTA on pulp cell apoptosis and proliferation in vitro. | | Author(s) | Moghaddame-Jafari S, Mantellini MG, Botero TM, McDonald NJ, Nör JE | | Institution | Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, Ann Arbor, MI 48109-1078, USA. | | Source | J Endod 2005 May; 31(5):387-91. | | MeSH | Aluminum Compounds
Analysis of Variance
Animals
Apoptosis
Calcium Compounds
Cell Proliferation
Cell Survival
Cells, Cultured
DNA Replication
Dental Pulp
Dental Pulp Capping
Drug Combinations
Flow Cytometry
Materials Testing
Mice
Odontoblasts
Oxides
Research Support, Non-U.S. Gov't
Root Canal Filling Materials
Silicates
| | Abstract | ProRoot Mineral Trioxide Aggregate (MTA) has been indicated as a pulp capping material. The purpose of this study was to evaluate the effect of tooth-colored (white) MTA on pulp cell apoptosis and cell cycle. Mouse odontoblast-like cells (MDPC-23) and undifferentiated pulp cells (OD-21) were exposed to 0 to 100 mg MTA for 24 h. Propidium iodide staining followed by flow cytometry demonstrated that MTA did not induce apoptosis of MDPC-23 or OD-21 (p > 0.05). Cell cycle analysis showed that MTA induced a modest (but significant) increase in the percentage of MDPC-23 in the S and G2 phases, and OD-21 in the S phase of cell cycle, as compared to untreated controls (p </= 0.05). In conclusion, MTA induced proliferation, and not apoptosis, of pulp cells in vitro. These findings suggest a potential mechanism to explain the regenerative effect observed in the dentin-pulp complex when MTA was used for direct pulp capping. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 15851935 |
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