| Title | Detection of anti-HLA antibodies with flow cytometry in needle core biopsies of renal transplants recipients with chronic allograft nephropathy. | | Author(s) | Martin L, Guignier F, Bocrie O, D'Athis P, Rageot D, Rifle G, Justrabo E, Mousson C | | Institution | Department of Pathology, School of Medicine, Dijon, France. laurent.martin@u-bourgogne.fr | | Source | Transplantation 2005 May 27; 79(10):1459-61. | | MeSH | Antibodies
Biopsy, Needle
Chronic Disease
Comparative Study
Enzyme-Linked Immunosorbent Assay
Feasibility Studies
Flow Cytometry
HLA Antigens
Histocompatibility Antigens Class I
Histocompatibility Antigens Class II
Humans
Kidney
Kidney Diseases
Kidney Transplantation
Research Support, Non-U.S. Gov't
Sensitivity and Specificity
Transplantation, Homologous
| | Abstract | The aim of this study was to assess the feasibility of detecting anti-HLA antibodies in eluates from needle core biopsies of renal transplants with chronic allograft nephropathy. Two methods of screening, the enzyme-linked immunosorbent assay (ELISA) and flow cytometry (FlowPRA) were compared. Twenty renal transplants with CAN were removed after irreversible graft failure. To assess the feasibility of detecting anti-HLA antibodies in small samples, needle core biopsies were sampled at the same place as surgical samples and at a second cortical area. Antibodies were eluted with an acid elution kit and anti-class I and class II IgG HLA antibodies detected using ELISA and flow cytometry. Flow cytometry was found to be more sensitive than ELISA for detecting anti-HLA antibodies in eluates from renal transplants with CAN (95% vs. 75% of positive cases). Detection of anti-HLA antibodies showed good agreement between surgical samples and needle core biopsies performed at the same place for anti-class I (80% vs. 65%, r=0.724 P<0.01) and anti-class II HLA antibodies (70% vs. 55%, r=0.827 P<0.01). In addition, differences in the detection of anti-class I HLA antibodies in needle core biopsies sampled at different sites suggests that immunization to class I donor antigen could be underestimated in needle core biopsy samples. These data indicate that anti-HLA antibodies can be detected in needle core biopsies from renal transplants. Provided further evaluation is done, elution might be a complementary method to detect anti-HLA antibodies when they are bound to the transplant. | | Language | eng | | Pub Type(s) | Evaluation Studies
Journal Article
| | PubMed ID | 15912120 |
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