| Title | Cytoplasmic inclusion bodies are detected by synthetic antibody in ciliated bronchial epithelium during acute Kawasaki disease. | | Author(s) | Rowley AH, Baker SC, Shulman ST, Fox LM, Takahashi K, Garcia FL, Crawford SE, Chou P, Orenstein JM | | Institution | Department of Pediatrics, Feinberg School of Medicine, Children's Memorial Hospital, Northwestern University, Chicago, IL 60611, USA. a-rowley@northwestern.edu | | Source | J Infect Dis 2005 Nov 15; 192(10):1757-66. | | MeSH | Antibodies, Monoclonal
Bronchi
Child, Preschool
Cytoplasm
Epithelium
Female
Humans
Immunohistochemistry
Inclusion Bodies
Infant
Male
Microscopy, Electron, Transmission
Mucocutaneous Lymph Node Syndrome
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
| | Abstract | BACKGROUND: In developed nations, Kawasaki disease (KD) is the most common cause of acquired heart disease in children. An infectious etiology is likely but has not yet been identified. We have previously reported that oligoclonal immunoglobulin A plasma cells infiltrate acute KD tissues and that synthetic KD antibodies detect a distinctive spheroidal antigen in acute KD ciliated bronchial epithelium.
METHODS: To further characterize the antigen in acute KD bronchi, we examined paraffin-embedded ciliated bronchial epithelium using light microscopy (LM) and transmission electron microscopy (TEM).
RESULTS: The spheroids observed by immunohistochemistry (IHC) are visualized as inclusion bodies with hematoxylin-eosin and nucleic acid stains and in methylene blue/azure II/basic fuchsin trichrome-stained plastic sections, suggesting the presence of both protein and nucleic acid. The structures visualized by LM correspond to homogeneous electron-dense perinuclear inclusion bodies (up to 1.4 microns in diameter) in ciliated bronchial epithelium from 4 patients with acute KD examined by TEM. Inclusion bodies were not present in control bronchial epithelium or in nonciliated cells.
CONCLUSIONS: The antigen detected in acute KD ciliated bronchial epithelium by IHC with synthetic KD antibodies resides in cytoplasmic inclusion bodies that are consistent with aggregates of viral proteins and associated nucleic acid and may derive from the etiologic agent of KD. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 16235174 |
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