| Title | Gingipain-specific IgG in the sera of patients with periodontal disease is necessary for opsonophagocytosis of Porphyromonas gingivalis. | | Author(s) | Gibson FC, Savelli J, Van Dyke TE, Genco CA | | Institution | Department of Medicine, Section of Infectious Diseases, Boston University Medical School, Boston, MA, USA. frank.gibson@bmc.org | | Source | J Periodontol 2005 Oct; 76(10):1629-36. | | MeSH | Adhesins, Bacterial
Adult
Antibodies, Bacterial
Case-Control Studies
Cysteine Endopeptidases
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunoglobulin G
Male
Neutrophils
Opsonin Proteins
Periodontitis
Phagocytosis
Porphyromonas gingivalis
Research Support, N.I.H., Extramural
| | Abstract | BACKGROUND: Porphyromonas gingivalis is a primary etiologic agent of generalized aggressive periodontitis (GAgP), and gingipains, a group of cysteine proteinases, are critical virulence factors expressed by this organism. GAgP patients develop specific antibodies to gingipains; however, the function of these antibodies in the clearance of P. gingivalis infection is poorly understood.
METHODS: In this study, we defined the levels of gingipain-specific antibodies in GAgP patient sera and examined the ability of gingipain-specific antibodies to facilitate opsonophagocytosis of P. gingivalis by human polymorphonuclear leukocytes (PMNs) using a fluorescent phagocytosis assay.
RESULTS: GAgP patient sera possessed elevated levels of P. gingivalis-, arginine-gingipain (Rgp)A-, RgpB-, and lysine-gingipain (Kgp)-specific IgG (Kgp > RgpA > P. gingivalis > RgpB). Adsorption of GAgP sera with P. gingivalis whole organisms, RgpA, RgpB, and Kgp conjugated to sepharose beads reduced opsonophagocytosis of P. gingivalis by PMNs.
CONCLUSIONS: Our studies demonstrate that GAgP patient sera possess elevated levels of P. gingivalis- and gingipain-specific IgG. Furthermore, we show that gingipain antibodies promote uptake of P. gingivalis by PMNs, and our data suggest that gingipain-specific antibodies may be important for the control of P. gingivalis infections. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 16253083 |
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