| Title | Effect of mineral trioxide aggregate on cytokine production by peritoneal macrophages. | | Author(s) | Rezende TM, Vargas DL, Cardoso FP, Sobrinho AP, Vieira LQ | | Institution | Departamento de Dentística Restauradora, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. | | Source | Int Endod J 2005 Dec; 38(12):896-903. | | MeSH | Aluminum Compounds
Animals
Calcium Compounds
Cell Survival
Cells, Cultured
Drug Combinations
Female
Fusobacterium nucleatum
Interferon Type II
Interleukin-10
Interleukin-12
Macrophages, Peritoneal
Male
Mice
Mice, Inbred C57BL
Mice, Inbred Strains
Oxides
Peptostreptococcus
Protein Subunits
Research Support, Non-U.S. Gov't
Root Canal Filling Materials
Silicates
Tumor Necrosis Factor-alpha
| | Abstract | AIM: To test the effect of two commercial brands of grey mineral trioxide aggregate (ProRoot and MTA-Angelus) on cytokine production by M1 and M2 inflammatory macrophages.
METHODOLOGY: M1 (from C57BL/6 mice) and M2 peritoneal inflammatory macrophages (from C57BL/6 IL12p40-/- mice) were obtained and cultured in vitro in the presence of MTA. The cellular viability and the production of tumour necrosis factor-alpha, interleukin (IL)-12 and IL-10 in response to stimulation with interferon-gamma and Fusobacterium nucleatum or Peptostreptococcus anaerobius were evaluated. Data were analysed by Mann-Whitney, Kruskal-Wallis and anova tests.
RESULTS: The cements did not interfere with cellular viability or with cytokine production by either type of macrophage. However, M2 macrophages produced higher levels of IL-10 when stimulated with F. nucleatum than M1 macrophages (P < 0.05).
CONCLUSIONS: The brands of MTA evaluated did not interfere in the cytokine response by M1 or M2 macrophages to the two bacteria tested. However, a difference in cytokine production between the two types of macrophages was found. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 16343117 |
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