| Title | Styraxoside A Isolated from the Stem Bark of Styrax japonica Inhibits Lipopolysaccharide-Induced Expression of Inducible Nitric Oxide Synthase and Cyclooxygenase-2 in RAW 264.7 Cells by Suppressing Nuclear Factor-kappa B Activation. | | Author(s) | Yun KJ, Min BS, Kim JY, Lee KT | | Institution | Department of Pharmaceutical Biochemistry and Kyung-Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung-Hee University. | | Source | Biol Pharm Bull 2007 Jan; 30(1):139-44. | | Abstract | In the present study, the effects of terpenes (styraxosides A and B) and lignans (egonol, masutakeside I, and styraxlignolide A) isolated from the stem bark of Styrax japonica SIEB. et ZUCC. (styracaceae) were evaluated on lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production by the RAW 264.7 macrophage cell line. Of the tested compounds, styraxoside A was found to most potently inhibit the productions of NO and PGE(2), and also significantly reduced the release of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta). Consistent with these observations, the protein expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and the mRNA expression levels of iNOS, COX-2, TNF-alpha and IL-1beta were found to be inhibited by styraxoside A in a concentration-dependent manner. Furthermore, styraxoside A inhibited the LPS-induced DNA binding activity of nuclear factor-kappaB (NF-kappaB). Taken together, our data indicate that styraxoside A inhibits LPS-induced iNOS, COX-2, TNF-alpha, and IL-1beta expressions through the down-regulation of NF-kappaB-DNA binding activity. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 17202674 |
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