Hong X, Long G, Jia H, Anqing L, Danzhou T
Simultaneous determination of residual stilbenes and stilbene metabolites in animal tissue by liquid chromatography-tandem mass spectrometry. [JOURNAL ARTICLE]
J Chromatogr B Analyt Technol Biomed Life Sci 2007 Mar 19.
A liquid chromatographic (LC)-tandem mass spectrometry (MS/MS) method was developed for simultaneous determination of stilbenes, diethylstilbestrol (DES), hexestrol (HEX), and dienoestrol (DEN) in animal tissue. Sample clean-up and analyte enrichment was performed by automated solid-phase extraction (ASPE) with a silica gel cartridge. Detection capabilities (CCbeta) related to the transition products of lowest abundance for the method were 0.04-0.45ngg(-1) in tissue and were achieved using atmospheric pressure chemical ionization (APCI) in negative mode. The use of an internal standard in combination with the simplified sample preparation led to a sensitive and reliable analytical method. The recovery level of the method was 84-108% for DES and DEN between 0.5 and 5ngg(-1), and 59-87% for HEX between 0.25 and 2.5ngg(-1).
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