| Title | Determination of synthetic hormones in animal urine by high-performance liquid chromatography/mass spectrometry. | | Author(s) | Rúbies A, Cabrera A, Centrich F | | Institution | Laboratori de l'Agència de Salut Pública de Barcelona, Edifici Peracamps, Avinguda Drassanes 13-15, 08001 Barcelona, Spain. arubies@aspb.es | | Source | J AOAC Int 2007 Mar-Apr; 90(2):626-32. | | Abstract | A method was developed for the extraction of stanozolol, taleranol, zeranol, hexestrol, dienestrol, ethynylestradiol, diethylstilbestrol, and trenbolone from animal urine. The analytes were extracted from the matrix by enzymatic hydrolysis, solid-phase extraction, and liquid-liquid extraction. Detection and quantitation were performed on a high-performance liquid chromatography system coupled to a triple quadrupole mass spectrometer. The identification criteria met the European Union regulations. Validation of this method established a decision limit between 0.2 and 0.9 microg/L and a detection capability between 0.3 and 1.0 microg/L, depending on the analyte. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 17474533 |
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