| Title | Short-term clearance of cell-free nucleic acids after first-trimester termination of pregnancy. | | Author(s) | Jeong YJ, Borgatta L, Kapp N, Peter I, Bianchi DW, Johnson KL | | Institution | Division of Genetics, Departments of Pediatrics and Obstetrics and Gynecology, Tufts-New England Medical Center, Boston, Massachusetts; Department of Obstetrics and Gynecology, Boston Medical Center, Boston. | | Source | Fertil Steril 2007 May 2. | | Abstract | To determine the kinetics of the short-term clearance of cell-free nucleic acids in maternal plasma, we conducted a prospective cohort study of 36 women who underwent first-trimester elective surgical termination. By using real-time polymerase chain reaction (PCR) amplification and measurement of the sex-determining region of the Y chromosome (SRY) gene (as a marker of fetal DNA), beta-hCG (a placental messenger RNA transcript), and glyceraldehyde phosphate dehydrogenase (GAPDH; as a marker of both total DNA and mitochondrial RNA), we found that cell-free nucleic acids in maternal plasma are not cleared within 90 minutes after the procedure, in contrast to the case of term delivery, in which levels decrease rapidly after birth. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 17481618 |
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