| Title | In vitro inhibition of Helicobacter pylori urease: biochemical and ultrastructural analysis. | | Author(s) | Nilius M, Bode G, Lehnhardt G, Malfertheiner P | | Institution | Department of Internal Medicine II, University of Ulm, Germany. | | Source | Eur J Clin Invest 1991 Oct; 21(5):551-7. | | MeSH | Ascorbic Acid
Copper
Helicobacter pylori
Hydroxamic Acids
Microscopy, Electron
Ultraviolet Rays
Urease
| | Abstract | Inhibition of H. pylori urease was studied by means of electron-microscopy and electrophoretic methods using different urease inhibitors, such as acetohydroxamic-acid (AHA), L-ascorbic acid (AsA), copper ions, a combination of L-ascorbic acid with copper ions and UV light. AHA in two different concentrations and AsA at a concentration of 0.1 mg ml-1 showed incomplete inhibition of H. pylori urease activity in our electrophoretic experiments. Only membrane-bound activity was inhibited with AHA but not the activity localized within the cytoplasma as demonstrated by electron-microscopy. AsA at a concentration of 0.5 mg ml-1 and the combination of copper ions (1 microgram ml-1) with AsA completely inhibited the urease activity as demonstrated by electron-microscopy and electrophoretic experiments. Cu2+ ions in high concentrations (100 micrograms ml-1) and UV light exposure for more than 4 h induced a complete disintegration of H. pylori. Electrophoresis showed no active protein after UV light exposure of 2 h. Different urease inhibitors tested in this study showed dose-dependent inhibitory effects on H. pylori urease in vitro. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 1752295 |
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