Montes-Moreno S, Roncador G, Maestre L, Martinez N, Sanchez-Verde L, Camacho F, Cannata J, Martinez-Torrecuadrada JL, Shen Y, Chan WC, Piris MA
Gcet1 (centerin), a highly restricted marker for a subset of Germinal Centre-derived lymphomas. [JOURNAL ARTICLE]
Blood 2007 Sep 26.
GCET1 (Germinal Centre B cell-Expressed Transcript-1) gene codes for a serpin expressed in Germinal Centre B-cells. Following the observation that Follicular Lymphoma cases exhibit an increased level of Gcet1 expression, when compared with follicular hyperplasia, we have characterized Gcet1 protein expression in human tissues, cell lines and a large series of lymphomas. To this end we have performed immunohistochemical and Western-Blot analyses using a newly generated monoclonal antibody that is reactive in paraffin-embedded tissues. Our results demonstrate that Gcet1 is expressed exclusively by neoplasms hypothetically to be arrested at the GC stage of differentiation, including FL, NLPHL and a subset of DLBCL, T/HRBCL and BL. Within these tumors, Gcet-1 protein expression is restricted to a subset of GC B cells, establishing the existence of a distinct heterogeneity among normal and neoplastic GC B cells. None of the other B-cell lymphomas, i.e., CLL, SMZL and MCL, was Gcet1-positive, which underlines the potential utility of Gcet1 expression in lymphoma diagnosis. The results of RNA and protein expression should prompt further investigation into the role of Gcet1 in regulating B cell survival.
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