| Title | CARF (collaborator of ARF) interacts with HDM2: Evidence for a novel regulatory feedback regulation of CARF-p53-HDM2-p21WAF1 pathway. | | Author(s) | Hasan MK, Yaguchi T, Harada JI, Hirano T, Wadhwa R, Kaul SC | | Institution | National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8562, Japan. | | Source | Int J Oncol 2008 Mar; 32(3):663-71. | | Abstract | We initially cloned CARF (collaborator of ARF), as a novel ARF-binding protein by a yeast interaction screen. It also interacts with p53 directly leading to ARF-independent enhancement of p53 function and in turn undergoes a negative feedback regulation. Herein we report that i) CARF interacts with HDM2 and undergoes degradation by an HDM2-dependent proteasome pathway, and ii) it acts as a transcriptional repressor of HDM2. By overexpression and silencing studies, we demonstrated that CARF exerts a vital control on the p53-HDM2-p21WAF1 pathway that is frequently altered in cancer cells. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 18292944 |
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