| Title | beta(1)- and beta(2)-adrenoceptor induced synaptic facilitation in rat basolateral amygdala. | | Author(s) | Abraham PA, Xing G, Zhang L, Yu EZ, Post R, Gamble EH, Li H | | Institution | Center for the Study of Traumatic Stress, Department of Psychiatry, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd., Bethesda, MD 20814, USA. | | Source | Brain Res 2008 Mar 8. | | Abstract | The expression and characteristics of beta-adrenoceptor subtypes (beta(1) and beta(2)) and their agonist actions on synaptic transmission in the basolateral amygdala (BLA) of the rat were examined using in situ hybridization, quantitative real-time PCR, Western blot analysis and field potential recording. In situ hybridization data revealed an intense distribution of beta(1)-and beta(2)-adrenoceptor mRNA in the BLA. Real-time PCR analysis of rat amygdala revealed significant transcriptional expression levels of both beta-adrenoceptors, with beta(2)-adrenoceptors outnumbering beta(1)-adrenoceptors in a ratio of 2.9 to 1. Bath application of the selective beta(1)-adrenoceptor agonist xamoterol hemifumarate (10 microM) facilitated the excitatory field synaptic potential evoked in the BLA by stimulation of the external capsule by 186.5+/-10.7% of control amplitude. In the presence of the selective beta(1)-adrenoceptor antagonist betaxolol hydrochloride (30 microM), the facilitating effects of field excitatory synaptic potential induced by the agonist were reduced to 126.1+/-2.3 % of control amplitude in the BLA. Bath application of the selective beta(2)-adrenoceptor agonist salmeterol (15 microM) facilitated the excitatory field synaptic potential evoked in the BLA by stimulation of the external capsule by 167.3+/-9.7 % of control amplitude. In the presence of the selective beta(2)-adrenoceptor antagonist ICI 118,551 HCl (30 microM), the facilitating effects of field excitatory synaptic potential induced by the agonist were reduced to 121.1+/-4.1 % of control amplitude in the BLA. These data suggest that beta-adrenoceptor mediated synaptic facilitation in the amygdala is mediated by both beta(1) and beta(2)-adrenoceptor activation. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 18396264 |
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