Quantitative determination of besifloxacin, a novel fluoroquinolone antimicrobial agent, in human tears by liquid chromatography-tandem mass spectrometry. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] Journal article | | Title | Quantitative determination of besifloxacin, a novel fluoroquinolone antimicrobial agent, in human tears by liquid chromatography-tandem mass spectrometry. | | Author(s) | Arnold DR, Granvil CP, Ward KW, Proksch JW | | Institution | Global Preclinical Development, Bausch & Lomb, 1400 North Goodman Street, Rochester, NY 14609, USA. | | Source | J Chromatogr B Analyt Technol Biomed Life Sci 2008 Apr 1. | | Abstract | A rapid and sensitive method was developed using high-performance liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) for the quantification of besifloxacin in human tears using sparfloxacin as the internal standard (IS). Besifloxacin was extracted from human tear samples using an ammonium formate buffer at pH 3.25. The method was validated over a concentration range of 2-2000ng/mL, with a total run time of less than 4min. The overall intra- and inter-day precision for this method was less than 6%. The method was used to measure besifloxacin concentrations in tear samples collected after topical ocular administration to humans; besifloxacin concentrations were 610+/-540mug/g (15min) and 1.60+/-2.28mug/g (24h). | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 18417430 |
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