| Title | Emulsified isoflurane produces cardiac protection after ischemia-reperfusion injury in rabbits. | | Author(s) | Rao Y, Wang YL, Zhang WS, Liu J | | Institution | Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei, China. | | Source | Anesth Analg 2008 May; 106(5):1353-9, table of contents. | | MeSH | Administration, Inhalation
Anesthetics, Inhalation
Animals
Coronary Vessels
Creatine Kinase
Cytoprotection
Disease Models, Animal
Fat Emulsions, Intravenous
Infusions, Intravenous
Isoflurane
L-Lactate Dehydrogenase
Ligation
Male
Microscopy, Electron, Transmission
Mitochondria, Heart
Myocardial Infarction
Myocardial Ischemia
Myocardial Reperfusion Injury
Myocardium
Protective Agents
Rabbits
Time Factors
| | Abstract | BACKGROUND: In this study, we examined the cardioprotective effects of parental emulsified isoflurane compared with inhaled isoflurane.
METHODS: Thirty-two rabbits were subjected to 30 min of myocardial ischemia induced by temporary ligation of the left anterior descending coronary artery followed by 3 h of reperfusion. Before left anterior descending coronary artery occlusion, the rabbits were randomly allocated into one of four groups (eight for each group): group C, no ischemia preconditioning treatment; group IS, inhaled isoflurane 1.1% end-tidal; group EI, a continuous infusion of 8% emulsified isoflurane to an end-tidal concentration of 0.64%; and group IN, a continuous infusion of 30% Intralipid started 30 min. Treatments were started 30 min before ischemia followed by a 15 min washout period for isoflurane groups. Myocardial infarct volume, lactate dehydrogenase, and creatine kinase levels were measured and changes in mitochondrial ultrastructure assessed after 3 h myocardial reperfusion.
RESULTS: Myocardial infarct size 3 h after reperfusion was lower in groups IS and EI compared with groups C and IN (20% +/- 8%, 18% +/- 8%, 39% +/- 6%, and 34% +/- 9%, respectively, P < 0.01). There were no differences in myocardial infarct size between groups IS and EI or between groups C and IN. Plasma lactate dehydrogenase and creatine kinase levels were lower in group IS (456 +/- 58 U/L and 1725 +/- 230 U/L) and group EI (451 +/- 54 U/L and 1686 +/- 444 U/L) 3 h after myocardial reperfusion compared with groups C (676 +/- 82 U/L and 2373 +/- 529 U/L; P < 0.01). Mitochondrial ultrastructure changes were less pronounced in groups IS and EI compared with group C.
CONCLUSIONS: Our results indicate that, in rabbits, i.v. emulsified isoflurane provides similar myocardial protection against ischemia-reperfusion injury as inhaled isoflurane. | | Language | eng | | Pub Type(s) | Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
| | PubMed ID | 18420844 |
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