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Isolation and purification of C3 from human plasma. Current protocols in immunology / edited by John E. Coligan ... [et al.] [Curr Protoc Immunol] Journal article

 
O'Rear LD, Ross GD 
Isolation and purification of C3 from human plasma. [Journal Article]
Curr Protoc Immunol 2001 May.:Unit 13.3.


The alternative pathway of complement shares its terminal components (C3 and C5 through 9) with the classical pathway, but has several unique components, including factors D, B, and P (properdin). This unit presents methods for assaying total alternative pathway activity and the activity of factors B and D. Radial immunodiffusion (RID) can also be used to measure factor D, B, and P concentrations.



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