| Title | Simultaneous separation of eight beta-adrenergic drugs using titanium dioxide nanoparticles as additive in capillary electrophoresis. | | Author(s) | Zhou S, Wang Y, De Beer T, Baeyens WR, Fei GT, Dilinuer M, Ouyang J | | Institution | College of Chemistry, Beijing Normal University, Beijing, P. R. China. | | Source | Electrophoresis 2008 Apr 25. | | Abstract | The analysis is described for separating seven beta-adrenergic blocking agents (atenolol, celiprolol, clorprenaline, fenoterol, metoprolol, propranolol, terbutaline) and clenbuterol (sympathomimetic beta-2 receptor stimulating agonist, decongestant and bronchodilator, illicit anabolic used in athletics) by CE with UV detection. In order to simultaneously separate all analytes, Tris-H(3)PO(4) solution was applied containing titanium dioxide nanoparticles (TiO(2) NPs) as BGEs. The effects of important factors, such as concentration of TiO(2) NPs, optimum pH, run buffer concentration, and separation voltage, were investigated so as to achieve best CE separation. The eight analytes could be well separated applying a separation voltage of 15 kV in 75 mM Tris-H(3)PO(4) buffer at a pH of 2.40, containing 6.0x10(-6) g/mL TiO(2) NPs. Under these optimal conditions, the RSDs for peak areas and for migration times were less than 2.7 and 2.3%, respectively. The detection limits were 0.1 mug/mL for celiprolol, 0.1 mug/mL for propranolol, 0.2 mug/mL for fenoterol, 1.0 mug/mL for atenolol, 1.0 mug/mL for clenbuterol, 1.0 mug/mL for clorprenaline, 1.0 mug/mL for metoprolol, and 1.0 mug/mL for terbutaline. The proposed method was successfully applied for the rapid CE determination of the frequently applied antihypertensive beta-blocking compounds atenolol, metoprolol, terbutaline, and propranolol in pharmaceutical tablets. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 18435493 |
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