| Title | The Effect of Starvation Stress on the Protein Profiles in Flexibacter chinensis. | | Author(s) | Raheb J, Naghdi S, Flint KP | | Institution | National Institute for Genetic Engineering and Biotechnology (NIGEB), Pazhoohesh Boulvard, Kilometers 17, P.O. Box: 1455-6343, Tehran, Iran. jam@nrcgeb.ac.ir. | | Source | Iran Biomed J 2008 Apr; 12(2):67-75. | | Abstract | Background: Analysis of many proteins produced during the transition into the stationary phase and under stress conditions (including starvation stress) demonstrated that a number of novel proteins were induced in common to each stress and could be the reason for cross-protection in bacterial cells. It is necessary to investigate the synthesis of these proteins during different stress conditions.
Methods: The changes in protein profile of Flexibacter chinensis at various stages of the starvation process and the other stresses were investigated using two-dimensional gel electrophoresis which has proven to be a powerful tool for investigation of the changes in protein profiles under such conditions.
Results: Most starvation proteins were synthesized during the early stationary phase and many of these proteins remained during long-term starvation. Some of these proteins were transiently synthesized. The sequencing result of one of the proteins showed that there was a 62.5% identity in 8 amino acids overlapped with the 5' residue of a 10 kDa chaperon protein which is known to be involved in the starvation stress response in other organisms.
Conclusion: There are many proteins synthesized in common with many stresses in Flexibacter chinensis. Some of these proteins must play a major role in the stability of the cell under different stresses. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 18506212 |
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