Fission yeast Pot1-Tpp1 protects telomeres and regulates telomere length. Science (New York, N.Y.) [Science] Journal article

Title	Fission yeast Pot1-Tpp1 protects telomeres and regulates telomere length.
Author(s)	Miyoshi T, Kanoh J, Saito M, Ishikawa F
Institution	Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
Source	Science 2008 Jun 6; 320(5881):1341-4.
MeSH	Amino Acid Sequence Carrier Proteins Chromatin Immunoprecipitation DNA, Fungal Immunoprecipitation Molecular Sequence Data Mutation Protein Binding Protein Structure, Tertiary Schizosaccharomyces Schizosaccharomyces pombe Proteins Telomerase Telomere Telomere-Binding Proteins Two-Hybrid System Techniques
Abstract	Telomeres are specialized chromatin structures that protect chromosomal ends. Protection of telomeres 1 (Pot1) binds to the telomeric G-rich overhang, thereby protecting telomeres and regulating telomerase. Mammalian POT1 and TPP1 interact and constitute part of the six-protein shelterin complex. Here we report that Tpz1, the TPP1 homolog in fission yeast, forms a complex with Pot1. Tpz1 binds to Ccq1 and the previously undiscovered protein Poz1 (Pot1-associated in Schizosaccharomyces pombe), which protect telomeres redundantly and regulate telomerase in positive and negative manners, respectively. Thus, the Pot1-Tpz1 complex accomplishes its functions by recruiting effector molecules Ccq1 and Poz1. Moreover, Poz1 bridges Pot1-Tpz1 and Taz1-Rap1, thereby connecting the single-stranded and double-stranded telomeric DNA regions. Such molecular architectures are similar to those of mammalian shelterin, indicating that the overall DNA-protein architecture is conserved across evolution.
Language	eng
Pub Type(s)	Journal Article Research Support, Non-U.S. Gov't
PubMed ID	18535244

Advertise on this site.