| Title | Impairment of T-regulatory cells in cord blood of atopic mothers. | | Author(s) | Schaub B, Liu J, Höppler S, Haug S, Sattler C, Lluis A, Illi S, von Mutius E | | Institution | Department of Pulmonary and Allergy, University Children's Hospital Munich, Munich, Germany. Bianca.Schaub@med.uni-muenchen.de | | Source | J Allergy Clin Immunol 2008 Jun; 121(6):1491-9, 1499.e1-13. | | MeSH | Adult
Antigens, CD
Cytokines
Female
Fetal Blood
Flow Cytometry
Forkhead Transcription Factors
Gene Expression
Humans
Hypersensitivity, Immediate
Infant, Newborn
Male
Mothers
RNA, Messenger
Receptors, Nerve Growth Factor
Receptors, Tumor Necrosis Factor
Reverse Transcriptase Polymerase Chain Reaction
T-Lymphocyte Subsets
T-Lymphocytes, Regulatory
Transforming Growth Factor beta
| | Abstract | BACKGROUND: Maternal atopy is a strong predictor for the development of childhood allergic diseases. The underlying mechanisms are ill defined, yet regulatory T (Treg) and T(H)17 cells may play a key role potentially shaping the early immune system toward a proallergic or antiallergic immune regulation.
OBJECTIVE: We examined T(H)1/T(H)2, Treg, and T(H)17 cell responses to innate (lipid A/peptidoglycan) and mitogen/adaptive (phytohemagglutinin/Dermatophagoides pteronyssinus 1) immune stimulation in cord blood from offspring of atopic/nonatopic mothers.
METHODS: Cord blood mononuclear cells from 161 healthy neonates (59% nonatopic, 41% atopic mothers) were investigated regarding Treg and T(H)17 cells (mRNA/surface markers), suppressive function, and proliferation/cytokine secretion.
RESULTS: Cord blood from offspring of atopic mothers showed fewer innate-induced Treg cells (CD4(+)CD25(+)high), lower mRNA expression of associated markers (glucocorticoid-induced tumor necrosis factor receptor-related protein/lymphocyte activation gene 3; P < .05), and a trend toward lower Forkhead box transcription factor 3 (Foxp3) expression. Treg cell function was impaired in mitogen-induced suppression of T effector cells in cord blood of offspring from atopic mothers (P = .03). Furthermore, IL-10 and IFN-gamma secretion were decreased in innate-stimulated cord blood of offspring from atopic mothers (P = .04/.05). Innate-induced IL-17 was independent of maternal atopy and highly correlated with IL-13 secretion.
CONCLUSION: In offspring of atopic mothers, Treg cell numbers, expression, and function were impaired at birth. T(H)17 cells were correlated with T(H)2 cells, independently of maternal atopy. | | Language | eng | | Pub Type(s) | Journal Article
Research Support, Non-U.S. Gov't
| | PubMed ID | 18539197 |
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