Hishinuma T, Suzuki K, Yamaguchi H, Yamagishi H, Koike T, Ohara S, Shimosegawa T, Mano N, Goto J
Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry. [JOURNAL ARTICLE]
J Chromatogr B Analyt Technol Biomed Life Sci 2008 Jun 12.
A rapid, simple and highly sensitive method was developed for the quantitative determination of lansoprazole and rabeprazole concentrations in 20muL of human serum using high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS). Analytes, along with an internal standard (lansoprazole deuterium derivatives), were separated using a mobile phase of acetonitrile/1mM ammonium formate (140/60, v/v) on a C18 analytical column and analyzed in the selected reaction-monitoring (SRM) mode. The lower limit of quantification was 0.25ng/mL. A good linear response was observed for each analyte (from 0.25ng to 2.5mug/mL). This method was useful for therapeutic drug monitoring and pharmacokinetic studies.
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