Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] Journal article | | Title | Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry. | | Author(s) | Hishinuma T, Suzuki K, Yamaguchi H, Yamagishi H, Koike T, Ohara S, Shimosegawa T, Mano N, Goto J | | Institution | Division of Pharmacotherapy, Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3, Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan. | | Source | J Chromatogr B Analyt Technol Biomed Life Sci 2008 Jun 12. | | Abstract | A rapid, simple and highly sensitive method was developed for the quantitative determination of lansoprazole and rabeprazole concentrations in 20muL of human serum using high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS). Analytes, along with an internal standard (lansoprazole deuterium derivatives), were separated using a mobile phase of acetonitrile/1mM ammonium formate (140/60, v/v) on a C18 analytical column and analyzed in the selected reaction-monitoring (SRM) mode. The lower limit of quantification was 0.25ng/mL. A good linear response was observed for each analyte (from 0.25ng to 2.5mug/mL). This method was useful for therapeutic drug monitoring and pharmacokinetic studies. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 18555753 |
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