Regeneration of tracheal epithelium utilizing a novel bipotential collagen scaffold. The Annals of otology, rhinology, and laryngology [Ann Otol Rhinol Laryngol] Journal article

Title	Regeneration of tracheal epithelium utilizing a novel bipotential collagen scaffold.
Author(s)	Tada Y, Suzuki T, Takezawa T, Nomoto Y, Kobayashi K, Nakamura T, Omori K
Institution	Department of Otolaryngology, School of Medicine, Fukushima Medical University, Fukushima, Japan.
Source	Ann Otol Rhinol Laryngol 2008 May; 117(5):359-65.
MeSH	Animals Collagen Disease Models, Animal Epithelium Follow-Up Studies Guided Tissue Regeneration Male Rats Rats, Sprague-Dawley Respiratory Mucosa Tissue Engineering Tissue Scaffolds Trachea Tracheal Stenosis Treatment Outcome Wound Healing
Abstract	OBJECTIVES: The purpose of the present study was to evaluate the effectiveness of a novel bipotential collagen scaffold as a bioengineered trachea for the regeneration of the tracheal epithelium. METHODS: The bipotential collagen scaffold was developed by conjugating a collagen vitrigel membrane to a collagen sponge in order to promote both epithelial cell growth and mesenchymal cell infiltration. The bipotential collagen scaffold was transplanted into tracheal defects in rats, and a conventional collagen sponge was implanted as a control model. Histologic examinations were undertaken to evaluate the results. RESULTS: The bioengineered trachea was covered with epithelium in the vitrigel model, but not in the control model, at 7 days after implantation. At 14 days after implantation, the bioengineered trachea was covered with epithelium involving the basal cell layer in the vitrigel model. At 28 days after implantation, a columnar ciliated epithelium was observed only in the vitrigel model. CONCLUSIONS: Our technique for trachea reconstruction using a novel bipotential collagen scaffold affords a feasible approach for accelerating epithelial regeneration on the intraluminal surface of the host tracheal defect.
Language	eng
Pub Type(s)	Comparative Study Journal Article Research Support, Non-U.S. Gov't
PubMed ID	18564533

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